abstract：:Human fibroblasts repair DNA damaged by bleomycin through both short-patch and long-patch pathways, mediated by an aphidicolin-resistant (beta) and aphidicolin-sensitive (delta) DNA polymerase respectively (DiGiuseppe, J.A. and Dresler, S.L. (1989) Biochemistry, 28, 9515-9520). Despite certain similarities, aphidicoli...

journal_title：Carcinogenesis

authors： DiGiuseppe JA,Hunting DJ,Dresler SL

更新日期：1990-06-01 00:00:00

abstract：:Several purified polychlorinated biphenyl (PCB) congeners with differing toxicity/tumor promotional activities in rat liver in vivo were tested for their effects on gap-junctional intercellular communication (GJIC) in cell strains and lines derived from human liver and skin. This in vitro assay is being developed to d...

journal_title：Carcinogenesis

authors： Swierenga SH,Yamasaki H,Piccoli C,Robertson L,Bourgon L,Marceau N,Fitzgerald DJ

更新日期：1990-06-01 00:00:00

abstract：:Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from ...

journal_title：Carcinogenesis

authors： Smith CA,Xu YZ,Swann PF

更新日期：1990-05-01 00:00:00

abstract：:A large number of chemical carcinogens require metabolic activation before they are biologically active. The metabolism of a few of these compounds has been investigated in cultured human tissues and cells and the metabolism has been compared with the metabolism in organs from experimental animals in which the compoun...

journal_title：Carcinogenesis

authors： Autrup H

更新日期：1990-05-01 00:00:00

abstract：:We have shown previously that the difference between C57BL/6J and C3H/HeJ male mice in their susceptibilities to chemically-induced liver tumors results predominantly from an allelic difference at the Hepatocarcinogen sensitivity (Hcs) locus. This locus modulates the rate of growth of preneoplastic liver lesions and m...

journal_title：Carcinogenesis

authors： Hanigan MH,Winkler ML,Drinkwater NR

更新日期：1990-04-01 00:00:00

abstract：:The reactive metabolites of the carcinogenic N-acetyl-2-aminofluorene (AAF) form adducts with the guanine base of DNA. The fluorescence emission characteristics of N-(deoxy-guanosin-8-yl)-N-acetyl-2-aminofluorene (dGuo-C8-AAF), N-(deoxyguanosin-8-yl)-2-aminofluorene (dGuo-C8-AF) and the two N7 = C8 imidazole ring-open...

journal_title：Carcinogenesis

authors： van Houte LP,Retèl J,Westra JG,van Grondelle R

更新日期：1990-04-01 00:00:00

abstract：:Both Snell dwarf mice (dw/dw) and their phenotypically normal heterozygotes (dw/+) were dosed with methylclofenapate (MCP) at daily intervals by gavage (25 mg/kg). Animals were killed at 12, 24, 36 and 72 h after the initial dose and the parameters of ploidy, nuclearity and DNA synthesis were measured in hepatocytes i...

journal_title：Carcinogenesis

authors： Styles JA,Kelly MD,Pritchard NR,Foster JR

更新日期：1990-03-01 00:00:00

abstract：:O-Methylation of catecholestrogens catalyzed by catechol-O-methyltransferase provides a major route for the rapid metabolic clearance of these steroids. However, the metabolic clearance rate of 4-hydroxyestradiol (4-OH-E2) is considerably lower than that of 2-hydroxyestradiol, although 2- and 4-hydroxycatecholestrogen...

journal_title：Carcinogenesis

authors： Roy D,Weisz J,Liehr JG

更新日期：1990-03-01 00:00:00

abstract：:Male Ah-responsive C57BL/10ScSn mice received a single dose of iron-dextran (600 mg Fe/kg) and were fed a diet containing 0.01% of the PCBs mixture Aroclor 1254 for up to 12 months. Iron caused a marked synergistic increase in liver size from 2 months with greatly elevated mitotic rates, numbers of basophilic foci and...

journal_title：Carcinogenesis

authors： Smith AG,Francis JE,Carthew P

更新日期：1990-03-01 00:00:00

abstract：:XB2 cells, a teratocarcinoma derived cell line of keratinocyte lineage, have been shown to proliferate and differentiate in low calcium medium (0.03 mM Ca2+) at a density of 500 cells/9.6 cm2 without the need for fibroblast feeder layers or conditioned medium. The degree of differentiation can be assessed by measureme...

journal_title：Carcinogenesis

authors： Hewitt SD,Duffy PA,Orton TC,Garner RC

更新日期：1990-03-01 00:00:00

abstract：:SENCAR mice are unusually sensitive to induction of papillomas and squamous cell carcinomas by initiation with 7,12-dimethylbenzanthracene (DMBA) and promotion by 12-O-tetradecanoylphorbol-13-acetate (TPA). Tumors induced by this protocol were tested for the presence of papillomavirus by immunohistochemistry, Southern...

journal_title：Carcinogenesis

authors： Sundberg JP,Binder RL,Maurer JK,Newmann EA,Cunniff JJ

更新日期：1990-02-01 00:00:00

abstract：:An in vivo method for assessment of DNA adduct formation and unscheduled DNA synthesis (UDS) in the esophagus of rats was devised. Small ventral incisions were made in the neck and upper abdomen regions of 6 week old F344 rats and ligation of the esophagus with thread at the two extreme ends performed to make an esoph...

journal_title：Carcinogenesis

authors： Qin XS,Nakatsuru Y,Kohyama K,Ishikawa T

更新日期：1990-02-01 00:00:00

abstract：:N-Hydroxy-2-acetylaminofluorene (N-OH-AAF), N-hydroxy-4'-fluoro-4-acetylaminobiphenyl (N-OH-FAABP) and N-hydroxy-4-acetylaminobiphenyl (N-OH-AABP) were compared for their initiation and promotion activity in the rat liver using a modified Solt-Farber system. N-OH-AAF, N-OH-FAABP and N-OH-AABP showed comparable initiat...

journal_title：Carcinogenesis

authors： van de Poll ML,van der Hulst DA,Tates AD,Meerman JH

更新日期：1990-02-01 00:00:00

abstract：:The aim of this study has been to define cytotoxic mechanisms that may cause clonal expansion in the liver of pre-carcinogenic cells. An in vitro model, which has been described previously, was used. Hepatocytes were isolated from carcinogen-treated rats and a high proportion of the cells were gamma-glutamyltranspepti...

journal_title：Carcinogenesis

authors： Stenius U,Rubin K,Gullberg D,Högberg J

更新日期：1990-01-01 00:00:00

abstract：:DNA strand breage in response to damage produced by UV (254 nm) radiation was characterized after permeabilization of diploid normal and xeroderma pigmentosum variant fibroblasts. The breakage reaction required ATP, Mg2+ and sucrose for maximal activity and was inhibited by 150 mM Na+ or K+ and 1 mM N-ethylmaleimide. ...

journal_title：Carcinogenesis

authors： Kaufmann WK,Briley LP

更新日期：1990-01-01 00:00:00

abstract：:Gangliosides from liver parenchymal and non-parenchymal cells were isolated from Fischer 344 rats that had been fed normal diet or a diet supplemented with 0.03% N-2-acetylaminofluorene (AAF) for 4 weeks. Gangliosides from liver cell fractions were characterized by an induction of both II3NeuAcIV3 alpha GalIV2FucGg4 a...

journal_title：Carcinogenesis

authors： Holmes EH

更新日期：1990-01-01 00:00:00

abstract：:Dose-related differences in the binding of DNA reactive intermediates for three environmentally important complex mixture particulate extracts and a well-studied carcinogen, benzo[a]pyrene (BaP), were examined in female C-57 mice following multiple topical treatments ranging from 1 to 120 mg/mouse. Particulate extract...

journal_title：Carcinogenesis

authors： Gallagher JE,Jackson MA,George MH,Lewtas J

更新日期：1990-01-01 00:00:00

abstract：:The catalytic activity of the nuclear enzyme poly(ADP-ribose) polymerase (NAD+ ADP-ribosyl transferase, EC 2,4,2,30) is totally dependent upon the presence of DNA strand breaks. Having isolated a full-length cDNA for the polymerase, we have now evaluated the effect of endogenously and exogenously induced DNA strand br...

journal_title：Carcinogenesis

authors： Bhatia K,Pommier Y,Giri C,Fornace AJ,Imaizumi M,Breitman TR,Cherney BW,Smulson ME

更新日期：1990-01-01 00:00:00

abstract：:The activation of 2-nitrofluorene (2-NF) to reactive intermediates that bind covalently to DNA, RNA and protein has been investigated both in vitro and in the rat in vivo. In vitro, such binding was catalyzed by the hepatic microsomal fraction, was NADPH dependent and could be inhibited by SKF 525A, an inhibitor of cy...

journal_title：Carcinogenesis

authors： Wierckx FC,Wedzinga R,Meerman JH,Mulder GJ

更新日期：1990-01-01 00:00:00

abstract：:The role of sulfation in the metabolic activation of the liver carcinogen N-hydroxy-4'-fluoro-4-acetylaminobiphenyl (N-OH-FAABP) in male rat liver was investigated. N-OH-FAABP was a substrate for sulfotransferases in vitro and sulfation was inhibited by the sulfotransferase inhibitors pentachlorophenol (PCP) and 2,6-d...

journal_title：Carcinogenesis

authors： van de Poll ML,Tijdens RB,Vondrácek P,Bruins AP,Meijer DK,Meerman JH

更新日期：1989-12-01 00:00:00

abstract：:Four monoclonal antibodies, PhIP-1, -2, -3 and -4, have been produced that bind to PhIP (2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine), a mutagenic aromatic amine produced in meat by cooking. The immunogen was an analog of PhIP conjugated to a carrier protein through the 2-amino group of PhIP, leaving the pyridine...

journal_title：Carcinogenesis

authors： Vanderlaan M,Watkins BE,Hwang M,Knize MG,Felton JS

更新日期：1989-12-01 00:00:00

abstract：:Expression of the human placental form of glutathione S-transferase (GST-pi), an enzyme proposed as a marker for human and experimental neoplasia, was immunohistochemically evaluated in 51 samples of 'normal' and diseased adult human uterine cervix. Five fetal uteri were also studied. GST-pi positivity was detected in...

journal_title：Carcinogenesis

authors： de Camargo JL,Tsuda H,Tatematsu M,Rodrigues MA,Yamada M,Tzuji K,Ito N

更新日期：1989-12-01 00:00:00

abstract：:The effect of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was studied using an immortalized human bronchial epithelial cell line, BEAS-2B, both in vivo and in vitro. The in vivo model consisted of tracheas reconstituted with an epithelium of BEAS-2B cells xenotransplanted into athymic nude mice. Int...

journal_title：Carcinogenesis

authors： Bonfil RD,Momiki S,Fridman R,Reich R,Reddel R,Harris CC,Klein-Szanto A

更新日期：1989-12-01 00:00:00

abstract：:Spindle cell carcinomas were identified using polyacrylamide gel electrophoresis and immunoblotting of proteins extracted from paraffin-embedded tissue sections. Immunohistochemistry using rabbit monospecific antisera against the mouse 55 kd keratin polypeptide also identified these tumors. A group of 53 SENCAR mice i...

journal_title：Carcinogenesis

authors： Klein-Szanto AJ,Larcher F,Bonfil RD,Conti CJ

更新日期：1989-11-01 00:00:00

abstract：:The lipoxygenase catalyzed epoxidation of 7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (BP-7,8-diol) was examined. Epoxidation of the BP-7,8-diol was catalyzed by 5- and 15-lipoxygenase in the presence of either arachidonic acid, gamma-linolenic acid, or 15-hydroperoxyeicosatetraenoic acid (15-HPETE). The anti-9,10-epoxy-7...

journal_title：Carcinogenesis

authors： Hughes MF,Chamulitrat W,Mason RP,Eling TE

更新日期：1989-11-01 00:00:00

abstract：:In this report we describe results showing that both arachidonic acid (20:4) release and prostaglandin (PG) synthesis in herpes simplex virus type 2 (HSV-2)-transformed tumor-derived rat fibrosarcoma (RFS) cells are inhibited by indomethacin (cyclooxygenase inhibitor). These data suggest that cyclooxygenase (prostagla...

journal_title：Carcinogenesis

authors： Heitman CR,Waite M,Kucera LS

更新日期：1989-11-01 00:00:00

abstract：:Serum selenium (Se) levels were measured before, during and after high-dose induction chemotherapy in 70 patients with acute non-lymphocytic leukemia (ANLL). Pre-treatment serum Se levels were lower in patients than in controls (0.082 +/- 0.033 micrograms/ml versus 0.097 +/- 0.035 micrograms/ml, P less than 0.01). Pre...

journal_title：Carcinogenesis

authors： Beguin Y,Bours V,Delbrouck JM,Robaye G,Roelandts I,Bury J,Fillet G,Weber G

更新日期：1989-11-01 00:00:00

abstract：:Catechol (1,2-dihydroxybenzene) is a major phenolic compound present in the co-carcinogenic fraction of cigarette tar. It has been shown to be a potent co-carcinogen with benzo[a]pyrene (BaP) in mouse skin. In this study we have examined the co-carcinogenic and co-initiating activities of catechol with racemic and ena...

journal_title：Carcinogenesis

authors： Melikian AA,Jordan KG,Braley J,Rigotty J,Meschter CL,Hecht SS,Hoffmann D

更新日期：1989-10-01 00:00:00

abstract：:The relationship between urinary metabolites and DNA damage in the urinary bladder epithelium of male and female rats was tested by alkaline elution assay after an intravesical injection of OPP or its urinary metabolites. 2-Phenyl-1,4-benzoquinone (PBQ) revealed a weak DNA-damaging activity in both sexes at 0.05-0.1%....

journal_title：Carcinogenesis

authors： Morimoto K,Sato M,Fukuoka M,Hasegawa R,Takahashi T,Tsuchiya T,Tanaka A,Takahashi A,Hayashi Y

更新日期：1989-10-01 00:00:00

abstract：:Four groups of six male Wistar rats (85 +/- 7 g) were fed a diet containing 0% (control) or 2% of the carcinogenic food antioxidant butylated hydroxyanisole (BHA) for 2 weeks. In this experiment, feeding 2% BHA is equivalent to a dose of 2.1 +/- 0.3 g BHA/kg/day. One 0% BHA and one 2% BHA-fed group of rats were daily ...

journal_title：Carcinogenesis

authors： Verhagen H,Furnée C,Schutte B,Hermans RJ,Bosman FT,Blijham GH,ten Hoor F,Henderson PT,Kleinjans JC

更新日期：1989-10-01 00:00:00

abstract：:Chemical carcinogens are generally activated enzymatically to electrophiles that form covalently bound carcinogen-DNA adducts. Detoxifying enzymes are competing with the activating enzymes for these procarcinogenic chemical substrates. Wide person-to-person variations in these two types of enzymatic activities are fou...

journal_title：Carcinogenesis

authors： Harris CC

更新日期：1989-09-01 00:00:00

abstract：:Human fibroblasts were embedded in microscopic-sized agarose beads and lysed in situ to produce chromosome-sized DNA trapped inside the beads. Conventional alkaline agarose gel electrophoresis of trapped DNA from cells damaged by X-rays before embedding gave mol. wts similar to those obtained with alkaline sucrose gra...

journal_title：Carcinogenesis

authors： Cleaver JE

更新日期：1989-09-01 00:00:00

abstract：:The role of urinary pH and Na+ concentration on the bladder carcinogenesis of o-phenylphenol (OPP) was examined in male F344 rats. The rats were given powdered diet containing 2% sodium o-phenylphenate (OPP-Na, group 1), 1.25% OPP plus 0.64% NaHCO3 (group 2), 1.25% OPP plus 0.32% NaHCO3 (group 3), 1.25% OPP plus 0.16%...

journal_title：Carcinogenesis

authors： Fukushima S,Inoue T,Uwagawa S,Shibata MA,Ito N

更新日期：1989-09-01 00:00:00

abstract：:There is indirect evidence to suggest that the excision-repair mechanism responsible for the removal of UV-induced thymine dimers may also play a role in the repair of cis-diamminedichloroplatinum(II) (cis-DDP)-induced DNA adducts in both bacteria and mammalian cells. It was hypothesized that UV dimers and cis-DDP add...

journal_title：Carcinogenesis

authors： Swinnen LJ,Fisher SG,Erickson LC

更新日期：1989-08-01 00:00:00

abstract：:An earlier study indicated that ozone (O3), a major pollutant in our atmosphere, acts as a carcinogen as well as a synergistic co-carcinogen with radiation in cultured hamster embryo cells and in mouse C3H10T1/2 cells. In this investigation we further characterize the oncogenic action of ozone, alone or in combination...

journal_title：Carcinogenesis

authors： Borek C,Ong A,Zaider M

更新日期：1989-08-01 00:00:00

abstract：:We previously reported a rapid production model for pancreatic carcinoma development in Syrian hamsters incorporating the principle of selection by resistance to cytotoxicity. In the present experiment, the efficacy of repeated augmentation pressure with regard to generation of pancreatic lesions in hamsters initiated...

journal_title：Carcinogenesis

authors： Mizumoto K,Kitazawa S,Ito S,Takashima Y,Tsutsumi M,Denda A,Konishi Y

更新日期：1989-08-01 00:00:00

abstract：:A new sensitive 32P-postlabeling assay for DNA adducts has been developed in which DNA is hydrolyzed initially by nuclease P1 and prostatic acid phosphatase instead of micrococcal nuclease and spleen phosphodiesterase as employed in previous postlabeling procedures. When DNA containing bulky adducts, X1, X2, .....Xn, ...

journal_title：Carcinogenesis

authors： Randerath K,Randerath E,Danna TF,van Golen L,Putman KL

更新日期：1989-07-01 00:00:00

abstract：:Cyclic 1,N2-propanodeoxyguanosine adducts are formed in vitro in DNA treated with alpha-acetoxy-N-nitrosopyrrolidine or its metabolite, crotonaldehyde. However, the in vivo formation of these cyclic adducts in DNA has not been demonstrated due to the lack of a sensitive detection method. In this study, a 32P-postlabel...

journal_title：Carcinogenesis

authors： Chung FL,Young R,Hecht SS

更新日期：1989-07-01 00:00:00

abstract：:Tumor induction with chronic feeding of methyl-donor-deficient diets has been well established; however, the biochemical and molecular mechanisms which predipose to tumorigenesis in this model are still not well understood. The purpose of the present investigation was to assess DNA damage and altered nucleotide metabo...

journal_title：Carcinogenesis

authors： James SJ,Yin L

更新日期：1989-07-01 00:00:00

abstract：:Carcinomas of the ethmoidal region of the nose are observed relatively frequently in cattle in several countries in tropical and subtropical latitudes. Viruses have been implicated as causative agents, but it has been observed that affected animals sometimes suffer from aflatoxicosis, and a role of aflatoxin B1 (AFB1)...

journal_title：Carcinogenesis

authors： Larsson P,Pettersson H,Tjälve H

更新日期：1989-06-01 00:00:00